Cat. No.
MABL-2067
Application
neutralising, therapeutic
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey, Mouse
Clone No.
ATR-107
From
Recombinant Antibody
Specificity
This antibody recognises both human and murine IL-21R extracellular domains, as both were used as targets during alternating rounds of phage display library panning. This antibody also binds to rat and cynomolgus monkey IL-21R, but does not cross-react with human IL-4Rα or IL-2Rβ.
Alternative Names
CD360; Ab01; Ab-01; ATR107; IL21R; Interleukin-21 receptor; IL-21 receptor; NILR; Novel interleukin receptor; Q9JHX3
UniProt
Q9HBE5
Immunogen
This antibody was isolated through phage display. The scFv parental clone (18A5), which binds human IL-21R, was isolated from the CS human scFv library, using BaF3 cells expressing human IL- 21R and biotinylated IL-21R-Fc fusion protein. Four phage display libraries were constructed based on 18A5, and variants with increased affinity for both human and murine IL-21R were identified, including ATR-107.
Application Notes
This antibody binds to both human and murine IL-21R; surface plasmon resonance analysis has revealed this antibody to have an affinity to human and cynomolgus monkey IL-21R of 2 nM, and to murine IL-21R of 16 nM (Vugmeyster et al, 2010). This antibody has been shown to neutralise the IL- 21-dependent proliferation of both activated human primary B and CD4+ T cells and mouse CD8+ T cells (Vugmeyster et al, 2010). Additionally, this antibody inhibits IL-21-induced phosphorylation of STAT3 in CD4+ T cells in a concentration-dependent manner (Zhu et al, 2013). In the MRL-Faslpr mouse model of lupus, administration of this antibody has been shown to reduce anti-dsDNA antibodies and IgG deposits in the kidney (Vugmeyster et al, 2010). This antibody did not induce cytokine storm-associated gene activation in human PBMCs in vitro, nor when administered to cynomolgus monkeys (Guo et al, 2010). When administered to humans as part of a phase I trial, this antibody was highly immunogenic, with a prolonged pharmacodynamic effect, but was observed to lead to the development of anti-drug antibodies in 75% of subjects (Hua et al, 2013).
Antibody First Published
Vugmeyster et al. In vitro potency, pharmacokinetic profiles and pharmacological activity of optimized anti-IL-21R antibodies in a mouse model of lupus MAbs. 2010 May-Jun; 2(3)： 335–346. PMID：20424514
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
