Cat. No.
MABL-2072
Application
Blocking, crystallization, neutralizing
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
FAb1.1
From
Recombinant Antibody
Specificity
The antibody is specific for human and murine IL-34. The antibody recognizes a largely continuous area at the junction of two IL-34 protomers. The epitope overlaps largely with binding epitopes responsible for CSF-1R binding.
Alternative Names
Interleukin-34; YW404.33.56
UniProt
Q6ZMJ4
Immunogen
The original antibody was generated by panning a Fab library against IL-34.
Application Notes
The crystal structure of the Fab fragment of the antibody in complex with human IL-34 was determined. The original antibody was reformatted as an IgG molecules and the binding affinity of both formats measured by BLI experiments (Kd= 17 nM and 120 pM respectively). The antibody blocked the biological activity of IL-34 in human monocyte viability. The antibody neutralized IL-34 in a proliferation assays (Ma et al., 2012; PMID: 22483114).
Antibody First Published
Ma et al. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure. 2012 Apr 4;20(4):676-87. PMID:22483114
Note on publication
The original paper shows the structure of IL-34 in complex with the Fab fragment of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
