Cat. No.
MABL-2076
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12-6-68
From
Recombinant Antibody
Specificity
This antibody binds human IL-4. Interleukin-4 is a cytokine secreted primarily by mast cells, T- cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. It induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It also induces expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1.
Alternative Names
IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
UniProt
P05112
Immunogen
The original antibody was generated by immunizing BALB/c mice subcutaneously with 25 µg of human IL-4 in 100 µl of 150 mM sodium chloride emulsified with 100 µl of complete Freund’s adjuvant.
Application Notes
The binding of this antibody to human IL-4 was confirmed with ELISA (PMID: 12825686).
Antibody First Published
Ehsani et al. Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants. Plant Mol Biol. 2003 May;52(1):17-29. PMID:12825686
Note on publication
Describes the expression of two murine antibodies in Agrobacterium tumefaciens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
