Cat. No.
MABL-2077
Application
inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
25D2-9
From
Recombinant Antibody
Specificity
This antibody binds human IL-4. Interleukin-4 is a cytokine secreted primarily by mast cells, T- cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. It induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It also induces expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1.
Alternative Names
IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; MP4.25D2.11; ATCC HB 9809
UniProt
P05112
Immunogen
The original antibody was generated by immunizing Lewis rat with 1ml of human IL-4. The humanized version of the antibody was created later by grafting CDRs from the original rat antibody onto human framework regions.
Application Notes
The binding characterization of this antibody for human IL-4 was done using ELISA. This antibody was also capable of inhibiting binding of radiolabeled hIL-4 to recombinant hIL-4 receptors expressed on the Jijoye CJ cell line. This antibody was also reported to inhibit induction of human growth hormone in the germline epsilon reporter assay (US5597710; US5863537).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
