Cat. No.
MABL-2078
Application
cytometric bead array, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AbAbS1-IL4
From
Recombinant Antibody
Specificity
This antibody binds human IL-4. Interleukin-4 is a cytokine secreted primarily by mast cells, T- cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. It induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It also induces expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1.
Alternative Names
IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
UniProt
P05112
Immunogen
The original antibody was generated by immunizing BALB/c mice with human interleukin-4.
Application Notes
This antibody can be used for detection of IL-4 using ELISA. A combination of AbAbS1- IL4 and AbAbS2-IL4 was used for the detection of IL-4 in a sandwich assay, where AbAbS1-IL4 was used as a coating antibody and AbAbS2-IL4 was used as detection antibody. This combination was also used in the generation of a cytometric bead array (CBA) kit for detection of IL-4. The sensitivity of detection for this antibody pair for the CBA kit was reported to be less than 0.5pg/mL (CN118388645).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
