Cat. No.
MABL-2093
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
61H7
From
Recombinant Antibody
Specificity
This antibody recognizes and binds interleukin-6 on the top of the four-helix bundle near the N and C termini. Here the VL domain sterically interferes with the C-terminal domain of IL-6R (residues 195–295). The overlap of the 61H7 epitope and the site I surface concentrates around a cavity occupied by both the HCDR3 loop of 61H7 and the IL-6R molecule.
Alternative Names
IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
UniProt
P05231
Immunogen
The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification.
Application Notes
This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274).
Antibody First Published
Blanchetot et al. Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies. J Biol Chem. (2016); 291(26); 13846-54. PMID:27129274
Note on publication
Describes the crystal structure and epitope characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
