Cat. No.
MABL-2103
Application
inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AUK12-20
From
Recombinant Antibody
Specificity
This antibody binds the human IL-6 receptor at a site that is different from the epitope recognized by Tocilizumab (PM-1). The presence of this antibody inhibits the binding of IL-6 to the human IL- 6R.
Alternative Names
Interleukin-6 receptor subunit alpha; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; Membrane glycoprotein 80; gp80; CD126
UniProt
P08887
Immunogen
The original antibody was generated by immunizing female BALB/c mice with 10.mu.g/mouse of the soluble IL-6R (SR 344) immunogen in Freund's complete adjuvant.
Application Notes
This antibody can be used to inhibit the binding of IL-6 to its receptor IL-6R. It is also reported to inhibit IL-6 functions. The binding characterization of this antibody was done using ELISA (US5795965). This antibody was also reported to inhibit IL-6 dependent tumor cell growth (PMID: 8152440). This antibody was used in a study wherein the combined effect of antigrowth factor receptor antibodies was tested on growth limitation of myeloma cells (PMID: 8120920).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
