Cat. No.
MABL-2104
Application
Blocking, therapeutic, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
D7715A7
From
Recombinant Antibody
Specificity
This antibody is specific for murine IL-6R.
Alternative Names
CD126; 15A7; IL-6Ra; IL6R; Interleukin-6 receptor subunit alpha; Il6ra; IL- 6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1
UniProt
P22272
Immunogen
This antibody was raised by immunising Lewis rats with OKT4 cells.
Application Notes
This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60% and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001).
Antibody First Published
Coulie et al. A monoclonal antibody specific for the murine IL-6-receptor inhibits the growth of a mouse plasmacytoma in vivo. Curr Top Microbiol Immunol. 1990;166:43-6. PMID:2073815
Note on publication
Describes the original generation of this antibody, and the characterisation of its ability to block IL-6 binding.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
