Cat. No.
MABL-2114
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SE2
From
Recombinant Antibody
Specificity
SE2 antibody is specific for IL-8RA and does not corss-react with IL-8RB. IL-8RA is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils.
Alternative Names
CXCR-1; C-X-C chemokine receptor type 1; CXC-R1; CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD181; interleukin 8
UniProt
P25024
Immunogen
This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
Application Notes
This antibody is recommended for the detection and analysis of IL-8RA expression. One group used it to identify IL-8RA on the surface of human granulocytes via FACS (Zahn et al., 1997). Importantly, SE2 antibody was specific to IL-8RA-expressing cells only. Subsequent flow cytometric analysis with this antibody led to the characterization of IL-8RA density on human leukocytes (Zahn et al., 1997).
Antibody First Published
Zahn et al. Chemoattractant receptors for interleukin-8 and C5a: expression on peripheral blood leukocytes and differential regulation on HL-60 and AML-193 cells by vitamin D3 and all- trans retinoic acid. Eur J Immunol. 1997 Apr;27(4):935-40. PMID:16144840
Note on publication
This article describes the generation and characterization of SE2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
