Cat. No.
MABL-2121
Application
functional assay, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
G23-8
From
Recombinant Antibody
Specificity
This antibody binds p19 subunit of mouse interleukin-23 (IL-23). IL-23A associates with IL-12B to form IL-23 interleukin which is a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro- inflammatory cytokine IL-17. Mouse IL-23 also induces strong proliferation of memory T cells. IL-23 is secreted by activated dendritic cells.
Alternative Names
IL23A; IL-23p19; IL23p19; IL23-p19; IL-23p19; SGRF; Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19
UniProt
Q9EQ14
Immunogen
The original antibody was generated by immunizing rats with recombinant mouse IL-23 heterodimer.
Application Notes
This antibody can neutralize the bioactivity of IL-23 with no effect on IL-12 p70 bioactivity. This antibody can be used for the detection of mouse IL-23 p19 in an ELISA (PMID: 18802055). This antibody was used in assay to determine the effect of anti-IL23p19 antibody on increase in IL-17 production after neutralization of IL-4 in T cells cultured with IL-4 DCs or with non transduced DCs (PMID: 17195211). This antibody was reported to inhibit lactic acid-induced enhancement of IL-17 production by OT-II mouse splenocytes that had been stimulated with the TLR ligand peptidoglycan (PGN) and bacillus Calmette-Gue´rin cell wall skeleton (BCG-CWS) (PMID: 18490716).
Antibody First Published
Sarkar et al. Interleukin-17 as a molecular target in immune-mediated arthritis: immunoregulatory properties of genetically modified murine dendritic cells that secrete interleukin-4. Arthritis Rheum. 2007 Jan; 56(1):89-100. PMID:17195211
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
