Cat. No.
MABL-2125
Application
Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AHT54
From
Recombinant Antibody
Specificity
AHT54 recognises a determinant close to the IL-2 binding site.
Alternative Names
CD25; Interleukin 2 receptor; IL-2R; IL2 receptor; IL-2 receptor; Tac antigen; p55; AHT-54; AHT 54
UniProt
P01589
Immunogen
BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.
Application Notes
AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992).
Antibody First Published
Osawa H et al. Studies on the formation of high-affinity IL-2 binding sites of an IL-2 receptor p55 + p75 heterodimeric complex: functional importance of a determinant on the p55 subunit defined by a monoclonal antibody AHT-107 Immunology Letters, 20 (1989) 205 - 212 Elsevie PMID:2469650
Note on publication
Describes the of binding monoclonal antibodies AHT107 and AHT54 to IL-2R and their inhibitory effect on IL-2 dependent proliferation of human T lymphoblasts.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
