Cat. No.
MABL-2133
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow
Clone No.
V2D2
From
Recombinant Antibody
Specificity
This antibody binds indolicidin (ILPWKWPWWPWRR), which is a potent antimicrobial peptide isolated from the neutrophil blood cells of cow. Indolicidin is a tryptophan-rich cationic broad spectrum antibiotic that can neutralize bacterial lipopolysaccharides and has immunomodulatory effects. Its antibacterial activity is realized inside the phagolysosomes of host neutrophils, outside of which it exhibits cytotoxicity. Some reports have demonstrated its interaction with various molecules like DNA, topoisomerase I, calmodulin, and ATP.
Alternative Names
CATHL4; Cathelicidin-4; Antimicrobial peptide; antibiotic
UniProt
P33046
Immunogen
The original antibody was generated by immunizing 8–10-week-old BALB/c mice with indolicidin conjugated to diphtheria toxoid.
Application Notes
The binding characterization of this antibody was done using ELISA. It was reported that this antibody could bind indolicidin with a binding affinity of KD = 56 nM and could block the antibacterial action of the peptide (PMID: 20837490).
Antibody First Published
Lomash et al. An antibody as surrogate receptor reveals determinants of activity of an innate immune peptide antibiotic. J Biol Chem. 2010 Nov 12;285(46):35750-8. PMID:20837490
Note on publication
Describes the generation of this antibody as a surrogate receptor for indolicidin to identify a motif that contained almost the entire antibiotic activity of native indolicidin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
