Cat. No.
MABL-2138
Application
RIA, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
calf, rodent, Human
Clone No.
Ab 123 (mAb3, CC9C10)
From
Recombinant Antibody
Specificity
This antibody binds an epitope on the B chain of calf, human and rodent insulin. Insulin is a peptide hormone produced by the beta cells of the pancreatic islets. It reduces blood glucose concentration by promoting its absorption from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes mellitus, a condition of high blood sugar level (hyperglycemia).
Alternative Names
INS; proinsulin
UniProt
P01317
Immunogen
The original antibody was generated by immunizing female BALB/c mice with calf insulin in Freund's complete adjuvant.
Application Notes
This antibody can be used to detect insulin in a sample using ELISA and radioimmunoassay (PMID: 2109009; 6193965). This antibody was used in the flow cytometric analysis of insulin occupied B cell receptors in VH125SD B6 mice (PMID: 26109644).
Antibody First Published
Schroer et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur J Immunol. 1983 Sep;13(9):693-700. PMID:6193965
Note on publication
Describes the generation of 18 different antibodies to map the antigenic determinants on the human insulin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
