Cat. No.
MABL-2137
Application
WB
Isotype
Engineer antibody
Species Reactivity
Human, Rodent
Clone No.
1D6-E3
From
Recombinant Antibody
Specificity
This antibody is specific for the IMPDH protein in both humans and rodents. IMPDH catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Alternative Names
Inosine-5'-monophosphate dehydrogenase 2; IMPDH2; IMP dehydrogenase 2; IMPD 2; IMPDH 2; IMPDH-II
UniProt
P12269
Immunogen
The antibody was generated by immunizing a mouse with the purified Chinese hamster IMPDH protein.
Application Notes
This antibody was used for a western blot on proteins from E.coli cells that were made to express IMPDH (Hager et al, 1995; pmid:7763314). HL-205 cells were used in a peroxidase-based western blot with this antibody (Glesne et al, 1991; pmid:7763314). HL-205 cells were used in a peroxidase-based western blot with this antibody (Glesne et al, 1991; pmid:1717828).
Antibody First Published
Glesne et al. Regulation of IMP Dehydrogenase Gene Expression by Its End Products, Guanine Nucleotides MOLECULAR AND CELLULAR BIOLOGY, Nov. 1991, p. 5417-5425 PMID:1717828
Note on publication
the regulation of IMP dehydrogenase (IMPDH), the rate-limiting enzyme of guanine nucleotide biosynthesis was studied, the effects of nucleosides, nucleotides, nucleotide analogs, or the IMPDH inhibitor mycophenolic acid (MPA) on the steady-state levels of IMPDH mRNA were examined.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
