Cat. No.
MABL-2136
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Listeria monocytogenes
Clone No.
3.3
From
Recombinant Antibody
Specificity
The antibody recognized epitopes within the leucine-rich repeat (LRR) region (amino acids 73- 248) and/ or the inter-repeat (IR) region(amino acids 249-342). It recognizes either an epitope overlapping the LRR and IR regions, or a conformational epitope not recognized by the antibody when one of the two regions is absent.
Alternative Names
Internalin B; InlB; Invasion protein InlB
UniProt
P0DQD3
Immunogen
The original antibody was generated by immunizing BALB/c mice with the protein InlB.
Application Notes
This antibody binds to InIB, a surface protein that mediates entry of Listeria monocytogenes into a wide variety of cell types. Binding affinity of the antibody was determined by ELISA and western blotting was used to confirm the specificity of the antibody. In particular, the antibody recognizes the region LRR and IR of INIB, indicating these regions of the protein are responsible for the entry into mammalian cells.
Antibody First Published
Braun et al. The 213-amino-acid leucine-rich repeat region of the listeria monocytogenes InlB protein is sufficient for entry into mammalian cells, stimulation of PI 3-kinase and membrane ruffling. Mol Microbiol. 1999 Oct;34(1):10-23. PMID:10540282
Note on publication
The authors identifies the region of InlB responsible for entry of the bacterium Listeria monocytogenes into a wide variety of cell types. This region is also responsible for the stimulation of signal transduction events. Eight monoclonal antibodies directed against InlB were raised and, of those, five inhibited bacterial entry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
