Cat. No.
MABL-2156
Application
crystallography, in vitro, SPR, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D2-57
From
Recombinant Antibody
Specificity
This antibody is specific for the LFA-1 I domain in a HA conformation.
Alternative Names
AL-57; CD11a; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain
UniProt
P20701
Immunogen
The original antibody was raised using human phage display library FAB-300. To screen the library purified locked-open HA, locked-closed LA, and wild-type I domains of LFA-1 were used.
Application Notes
The original antibody was used for an ELISA on LFA-1 HA I domain. This antibody was used for immunofluorescence on K562 cells with different LFA-1 conformations. Furthermore this antibody was used for flow cytometry on K562 cells with different LFA-1 conformations (Huang et al., 2006; PMID:16888085), and on IL-15-cultured T lymphocytes (Shimaoka et al., 2006; PMID:16963559). This antibody was used for an ICAM-1 blocking assay on HA cells, to determine the effect of the antibody to inhibit ligand binding. The antibody was used for a PHA-induced lymphocyte proliferation assay on PBMCs, to determine the effect of the antibody on these cells. It did indeed block PHA-induced lymphocyte proliferation (Huang et al., 2006; PMID:16888085). This antibody was used for surface plasmon resonance on LFA-1 I domain to determine its binding affinity (Shimaoka et al., 2006; PMID:16963559). The structure of this antibody was determined using x-ray crystallography (Zhang et al., 2009; PMID:19805116).
Antibody First Published
Huang et al. Identification and characterization of a human monoclonal antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function- associated antigen-1 J Leukoc Biol. 2006 Oct;80(4):905-14. PMID:16888085
Note on publication
The original paper reports the identification and characterization of a human antibody AL-57, which binds to the alpha(L) I domain in a HA but not LA conformation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
