Cat. No.
MABL-2155
Application
in vitro, in vivo, SPR, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
339.1
From
Recombinant Antibody
Specificity
This antibody is specific for alpha-5 beta-1
Alternative Names
Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29; Itga5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; CD49e; VLA-5
UniProt
P11688; P09055
Immunogen
The original antibody was raised by immunizing a Female Sprague-Dawley rat with alpha-5 beta-1 purified from mouse placenta or with a mouse alpha-5 beta-1-Fc fusion protein
Application Notes
The original antibody was used for an ELISA on alpha-5 beta-1-Fc fusion protein. The original antibody was used for surface plasmon resonance on alpha-5 beta-1-Fc. It showed a binding affinity of 0.59 nM. This antibody was used for an Annexin V cell death assay. Furthermore this antibody was used for immunohistochemistry on Xenograft tumours derived from cells inoculated subcutaneously in ICR-SCID or SCID-Beige mice (Bhaskar et al., 2007; PMID:18042290). This antibody was used to test the effect of alpha-5 and beta-1 integrin blockers on OvCa cell lines HeyA8 and SKOV3ip1. Further this antibody was used for in vivo testing of alpha-5 and beta-1 integrin blocking antibodies using the HeyA8 xenograft model of OvCa metastasis. It showed a decrease in the number of tumours in the subjects (Kenny et al., 2014; PMID:25202979).
Antibody First Published
Bhaskar et al. A function blocking anti-mouse integrin alpha-5 beta-1 antibody inhibits angiogenesis and impedes tumor growth in vivo. J Transl Med. 2007 Nov 27:5:61. PMID:18042290
Note on publication
The original paper demonstrates that 339.1, like volociximab, exhibits potent anti- α5β1 activity and confirms that inhibition of integrin α5β1 impedes angiogenesis and slows tumor growth in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
