Cat. No.
MABL-2154
Application
crystallography, in vivo, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Sheep, Human
Clone No.
AQC2
From
Recombinant Antibody
Specificity
This antibody is specific for VLA-1
Alternative Names
hAQC2; huAQC2; VLA-1; CD49a; ITGA1; Laminin and collagen receptor; CD49 antigen-like family member A
UniProt
P56199
Immunogen
The original antibody was raised by immunizing a mouse with human VLA-1.
Application Notes
The structure of the humanized version of this antibody was determined using x-ray crystallography. The humanized antibody was used for an ELISA on human alpha 1 beta 1 I domain (Karpusas et al., 2003; PMID:12662928). This antibody was used for a western blot on alpha-1 integrin of different animals, showing that this antibody could also bind sheep alpha-1 integrin. This antibody was used as an aerosol to prevent allergic reactions in the lungs of sheep. It only showed an effect on the late airway responses and no effect on the initial allergic reaction (Abraham et al., 2004; PMID:14578216).
Antibody First Published
Gotwals et al. Divalent Cations Stabilize the alpha-1 Beta-1 Integrin I Domain Biochemistry. 1999 Jun 29;38(26):8280-8. PMID:10387073
Note on publication
The original paper generated and characterized a panel of antibodies directed against the alpha-1 I domain.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
