Cat. No.
MABL-2158
Application
FACS, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Y2/51
From
Recombinant Antibody
Specificity
This antibody recognises integrin beta-3 which is a receptor for multiple ligands, such as laminin, fibronectin, prothrombin and others. It is involved in platelet-platelet interactions leading to a blood clot creation. Mutations in this protein are known to be a cause of Glanzmann thrombasthenia, the most common genetic disease of platelets.
Alternative Names
Integrin b3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; GP3A; INGRB3; ITG B3; ITGB3; Integrin β3
UniProt
P05106
Immunogen
This antibody was raised by immunising Balb/c mice with PHA-stimulated peripheral blood cells and subsequent fusing spleen cells and NSl myeloma cells.
Application Notes
This antibody has been extensively used as a marker of megakaryocytes. It is very effective in the immunohistochemical detection of platelets and megakaryocytes in formalin-fixed tissue samples as well as via Western blot and was also recommended for the identification of blood clots in blood vessels (Gatter et al., 1988). Y2/51 antibody has been also found useful in characterisation of other cells and tissues. For instance, one group used it (in concert with other antibodies) to compare immunohistochemically fibroblasts from normal and ruptured human anterior cruciate ligaments for tissue engineering purposes (Brune et al., 2007). Finally, another group performed immunofluorescence staining of HCT15 cells with this antibody and noted increased Integrin beta-3 expression in butyrate-preselected cells in a study on the acquisition of butyrate-resistance by colon cancer cells (Serpa et al., 2010).
Antibody First Published
Gatter et al. The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology. 1988 Sep;13(3):257-67. PMID:3192191
Note on publication
This article describes generation and characterisation of Y2/51 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
