Cat. No.
MABL-2159
Application
IP, Block, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
FIB27
From
Recombinant Antibody
Specificity
FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta-7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell- extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.
Alternative Names
FIB-27; FIB 27; Fib-27; Fib27; Fib 27; fib27; fib-27; fib 27; Integrin b7; integrin b-7; ITGB7; Gut homing receptor beta subunit
UniProt
P26010 (human); P26011 (mice)
Immunogen
FIB27 was prepared by immunizing rats with TK1 cells.
Application Notes
FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin- mediated cell adhesion.
Antibody First Published
Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153:3847 1994 PMID:7523506
Note on publication
Describes the use of FIB27 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
