Cat. No.
MABL-2161
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
107
From
Recombinant Antibody
Specificity
This antibody binds to the MIDAS face of the A domain from integrin CD11b.
Alternative Names
CD11b; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; leukocyte adhesion receptor MO1; Neutrophil adherence receptor; complement receptor 3
UniProt
P11215
Immunogen
Wild type (WT) recombinant CD11b.
Application Notes
The antibody binds specifically to integrin α-M, a protein subunit of the α-M- β-2 molecule, which is expressed on the surface of leukocytes. Integrin α-M is involved in the adhesive interaction between molecules of the innate immune system, and plays a role in the uptake of complement- coated particles. The monoclonal antibody 107 has a blocking activity by binding to the metal-ion- dependent-adhesion site, favouring a low affinity conformation of the antigen.
Antibody First Published
Li R, Haruta I, Rieu P, Sugimori T, Xiong JP, Arnaout MA. Characterization of a conformationally sensitive murine monoclonal antibody directed to the metal ion-dependent adhesion site face of integrin CD11b. J Immunol. 2002 Feb 1;168(3):1219-25. PMID:11801658
Note on publication
Describes the binding of the murine monoclonal antibody 107 to integrin CD11b. The antibody mimics the binding of divalent cations to the integrin, thereby inhibiting the association of CD11b to 11bA, blocking phagocytosis by human neutrophils.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
