Cat. No.
MABL-2164
Application
crystallography, IP, RIA, SPR
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A6
From
Recombinant Antibody
Specificity
This antibody is specific for Interferon gamma receptor 1.
Alternative Names
IFNGR1; CD119; CDw119; IFN-gamma-R1; IFN-gamma-R-alpha; Interferon gamma receptor alpha-chain
UniProt
P15260
Immunogen
The original antibody was raised by immunizing a Balb/c mouse with Membrane-enriched fractions of ~5x 10^10 human Raji cells affinity purified on rhuIFN-y coupled to Affigel-10.
Application Notes
The original antibody was used for a radioimmunoassay on 125I-labeled rhuIFN-gamma (Aguet et al., 1987; PMID:2435837, Bridges et al., PMID:8738212). This antibody was able to immunoprecipitate huIFN-gamma from lysates of enriched Raji cell membranes (Aguet et al., 1987; PMID:2435837). The structure of the original antibody was determined using x-ray crystallography (Sogabe et al., 1997; PMID:9367779, Hofstädter et al., 1999; PMID:9878445). The scFv version of this antibody was used for surface plasmon resonance on intact IFNyR (Bridges et al., PMID:8738212, Hofstädter et al., 1999; PMID:9878445).
Antibody First Published
Aguet and Merlin. Purification of human gamma interferon receptors by sequential affinity chromatography on immobilized monoclonal antireceptor antibodies and human gamma interferon J Exp Med. 1987 Apr 1;165(4):988-99. PMID:2435837
Note on publication
In the original paper mAbs against human IFN-gamma (huIFN-gamma) receptors were obtained by immunizing a BALB/c mouse with eluates from immobilized recombinant huIFN-gamma (rhuIFN-gamma) on which lysates of enriched Raji cell membranes had been adsorbed.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
