Cat. No.
MABL-2163
Application
Blocking, in vivo, neutralization, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
XMG1.2
From
Recombinant Antibody
Specificity
This antibody is specific for mouse interferon gamma.
Alternative Names
IFN gamma; IFN-gamma; interferon-γ; IFN-γ; IFNG; IFG; IFI; IMD69
UniProt
P01580
Immunogen
The original antibody was generated by immunizing a Lewis rat with soluble recombinant mouse IFN-γ.
Application Notes
The original antibody (rat IgG1, κ) was effective in both ELISA and two blocking bioassays (Cherwinski et al., 1987; PMID: 2960769). This antibody was used successfully in flow cytometry (Sander et al., 1993; PMID: 8288874). This antibody was used in vivo to block and neutralize IFN-γ (Suzuki and Joh, 1994; PMID: 8202451) (Beatty and Paterson, 2001; PMID: 11160282).
Antibody First Published
Cherwinski et al. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987 Nov 1;166(5):1229-44. doi: 10.1084/jem.166.5.1229 PMID:2960769
Note on publication
The original publication explores the lymphokine synthesis patterns of T cell clones, distinguishing between two types (Th1 and Th2) based on mRNA hybridization, revealing differences in the production of specific cytokines and providing insights into the functional specificity of these clones using monoclonal antibodies and bioassays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
