Cat. No.
MABL-2175
Application
crystallisation, FACS, IP, SPR, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
HL4E10
From
Recombinant Antibody
Specificity
HL4E10 was found to be specific for JAML, as this protein was identified as the ligand for the mAb from immunoprecipitation (Witherden et al, 2010). Biochemical binding studies showed that HL4E10 bound the JAML D2 Ig domain, which was corroborated with a recent crystal structure of the JAML ECD and a HL4E10 Fab fragment (Verdino et al, 2011). HL4E10 has an affinity for JAML of 8 nM, characterised by SPR.
Alternative Names
MICA1; Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1
UniProt
Q86YT9
Immunogen
An Armenian hamster was immunised with the 7-17 dendritic epidermal T cell line. Splenocytes were obtained from immunised hamsters and fused with the X63Ag8.653 myeloma cell line to generate a hybridoma.
Application Notes
HL4E10 has been used to detect JAML by both IP and FC (Witherden et al, 2010). IF labelling showed that JAML colocalises with the T cell receptor.
Antibody First Published
Witherden et al. The junctional adhesion molecule JAML is a costimulatory receptor for epithelial gammadelta T cell activation. Science. 2010 Sep 3;329(5996):1205-10. PMID:20813954
Note on publication
Describes the characterisation of JAML as a gammadelta T cell surface marker. mAb HL4E10 was produced and characterised herein as a JAML activator.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
