Cat. No.
MABL-2197
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MIMA-8
From
Recombinant Antibody
Specificity
MIMA-8 was shown to be specific for the Jsb epitope of the Kell protein using flow cytometry, whereby the mAb agglutinated Jsb+ , but not Jsa+Jsb-, erythrocytes (Chu et al, 1999). These erythrocytes also lacked other high-incidence Kell protein antigens. The Kell protein is a cell-surface metalloprotease, and constitutes one of the major blood antigenic systems of humans.
Alternative Names
CD238; Kell protein; Kell blood group glycoprotein homolog; Cluster of Differentiation 238
UniProt
Q9EQF2
Immunogen
Murine antibodies were produced by immunising BALB/c mice with the mouse erythroleukaemic (MEL) cell line stably expressing high levels of wildtype, full-length human Kell protein. This overexpression allowed several epitopes of Kell protein to serve as the immunogen, resulting in identification of several anti-Kell mAbs with different specificities, of which MIMA-8 targeted the Jsb antigen. Splenocytes were obtained from immunised mice and fused with the P3X63Ag8.653 myeloma cell line to produce a hybridoma.
Application Notes
MIMA-8 was shown, by flow cytometry, to react to the Jsb epitope of Kell protein (Chu et al, 1999). As a result, MIMA-8 could be used as a diagnostic reagent for cell typing of patient blood samples, or as a research tool. Notably, the Jsb epitope is known to be located close to the Kell enzymatic active site, and thus MIMA-8 could be used to study the structure-function relationship at this region.
Antibody First Published
Chu et al. Production and characterization of anti-Kell monoclonal antibodies using transfected cells as the immunogen British Journal of Haematology, 1999, 106, 817-823 PMID:10468879
Note on publication
Describes the generation of murine anti-Kell antigen mAbs, including MIMA-8. The variable domains of MIMA-8 were sequenced by obtaining cDNA, using RT-PCR of total RNA extracted from the hybridoma cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
