Cat. No.
MABL-2198
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C11
From
Recombinant Antibody
Specificity
This antibody recognizes the human keratin, types 4, 5, 6, 8, 10, 13, and 18. Because of its wide range in keratin specificity, it often is referred to in literature as pan-keratin.
Alternative Names
Pan-keratin; Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; Type-II keratin Kb5
UniProt
P13647
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing mice against human cytoskeleton
Application Notes
This original IgG1 antibody can be used to detect human keratin. Its specificity has been confirmed using immunofluorescence (Fehm et al., 2002; PMID: 12114406). One group used immunofluorescence to test if circulating epithelial cells were malignant (Fehm et al., 2002; PMID: 12114406), which was later built upon with the discovery that these cells remained in circulation until many years after treatment (Meng et al., 2004; PMID: 15623589). Another group used the antibodies with immunofluorescence and immunohistochemistry to visualize the progression of breast cancer once it had breached the epithelial cell layer (Khalil et al., 2017; PMID: 28894989). A final group used this antibody with immunofluorescence and western blotting to research the apoptotic response of keracinocytes to ultraviolet-B light (Mei, Lian, 2011; PMID: 21362287).
Antibody First Published
Kovarík et al. Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread Int J Cancer Suppl. 1988;3:50-5 PMID:2463228
Note on publication
Is the first mention of the antibody by the same lab that raised it, and describes its usuage
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
