Cat. No.
MABL-2200
Application
ISB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IAD7
From
Recombinant Antibody
Specificity
This antibody is specific for keratin with an increased affinity for native keratin over sulfur mustard-exposed (SM-exposed) keratin.
Alternative Names
UniProt
Immunogen
The original antibody was selected from existing phage display libraries.
Application Notes
The binding characteristics of this antibody were observed in an ELISA and immunoslotblot (ISB) assay. Increased exposure levels of keratin resulted in a decreased emission, indicating decreased stability of the antibody-keratin interactio; the formation of adducts or crosslinks disrupts effective antibody binding (Bikker et al., 2007; PMID: 17539823).
Antibody First Published
Bikker et al. Detection of sulfur mustard adducts in human callus by phage antibodies Chem Biol Drug Des. 2007 May;69(5):314-20. doi: 10.1111/j.1747-0285.2007.00504.x PMID:17539823
Note on publication
The original publication explores the application of phage display in the detection of sulfur mustard adducts in human callus, demonstrating the successful use of antibody phage display for immunodiagnostics of a chemical warfare agent. Furthermore, the publication describes the generation and characterization of clone IAD7.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
