Cat. No.
MABL-2207
Application
IP, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KE9
From
Recombinant Antibody
Specificity
This antibody is specific for human KRT19. KRT19 is involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Alternative Names
Keratin-19; K19; CK-19; Cytokeratin-19; Keratin type I cytoskeletal 19
UniProt
P08727
Immunogen
This antibody was raised panning against a library created by immunizing llamas with either breast cancer biopsy lysates.
Application Notes
To help identify the target of this antibody immunoprecipitation was preformed on T47D cells using the VHH version of this antibody bound to streptavidin beads. Further the ability of this antibody to be used for breast cancer research was assayed using immunohistochemistry. In short, breast cancer tissue was stained using the VHH version of this antibody. Finally to characterize this antibody. An ELISA was preformed using the VHH version of this antibody on KRT19 (Even-Desrumeaux et al, 2012; pmid22772166).
Antibody First Published
Desrumeaux et al. Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches. Mol Biosyst. 2012 Sep;8(9):2385-94. PMID:22772166
Note on publication
phage display libraries from animals immunized with breast cancer biopsies were generated. These libraries were used to isolate sdAbs against known and relevant antigens such as HER2, or several cancer-specific sdAbs against unknown targets.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
