Cat. No.
MABL-2217
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Ab835
From
Recombinant Antibody
Specificity
The antibody is specific for human LAG-3. The antibody does not cross react with cynomolgus LAG-3.
Alternative Names
CD223; LAG3; FDC; Lymphocyte activation gene 3 protein
UniProt
P18627
Immunogen
Application Notes
The antibody is a humanized version of the mouse antibody mab7c. The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50=0.12 nM). The binding activity of the humanized antibody was tested using the hLAG-3 positive cell line, and the results showed that the antibody was positive for hLAG-3. The binding activity of the antibody and hLAG-3 positive cells was EC50 =0.41 nM. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.52 nM). The antibody blocked the binding of MHCII and human LAG-3 with IC50 = 2.27 nM and it prevented LAG-3 and MHCII from binding to activate human T cell activity (release IL2) (EC50=1.74ug/ml) (CN110172099).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
