Cat. No.
MABL-2216
Application
Blocking, in vivo, SPR
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Ab2317
From
Recombinant Antibody
Specificity
The antibody is specific for human LAG-3. The antibody does not detect cynomolgus LAG-3 or murine LAG-3.
Alternative Names
CD223; LAG3; FDC; Lymphocyte activation gene 3 protein
UniProt
P18627
Immunogen
Application Notes
The antibody is a humanized version of mab23. The antibody had an activity EC50 of 0.34 μg/ml for activating human T cells to release IL-2. Further, the antibody activates human DC cell to stimulate human T cell activity (EC50 of 0.21nM), as shown by MLR assay. The humanized antibody efficacy in vivo was evaluated of the present invention. Tumors treated in mice showed an inhibition in tumor growth when treated with a combination of the antibody and PD-1 antibody. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.2 nM). The antibody showed a good blocking activity. The antibody was used in combination with an anti PD-1 antibody (Nivo or Pem) into a bispecific antibody (CN112079925).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
