Cat. No.
MABL-2221
Application
Blocking, in vitro, SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B5D1
From
Recombinant Antibody
Specificity
The antibody is specific for human LAG-3. The antibody cross-reacts with cynomolgus LAG-3.
Alternative Names
CD223; LAG3; FDC; Lymphocyte activation gene 3 protein
UniProt
P18629
Immunogen
Application Notes
The humanized version of the antibody was constructed. The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding activity of the humanized antibody to LAG3 was EC 50= 63.3 ng/ml. The binding affinity of the humanized antibody was measured by surface plasmon resonance (Kd= 6.10^-10 M). This antibody was used for detection of LAG-3 expressed on CHO cells by flow cytometry. The antibody blocked the binding of LAG-3 antigen and MHC-II molecule (IC50= 0.152 ug/ml). The antibody could effectively activate the activity of antigen-specific T cells, EC50 of in vitro activity test results of B5D1 antibody equal to 1.25 ug/ml (CN110606892).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
