Cat. No.
MABL-2220
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ADI-31798
From
Recombinant Antibody
Specificity
This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells.
Alternative Names
CD223; LAG3; FDC; Lymphocyte activation gene 3 protein
UniProt
P18627
Immunogen
The original antibody was isolated from a yeast display library by two rounds of screening against human LAG-3.
Application Notes
This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
