Cat. No.
MABL-2223
Application
BLI, Blocking, inhibition, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
rhesus macaque, Human
Clone No.
G8
From
Recombinant Antibody
Specificity
The antibody binds to human and rhesus macaque LAG-3.
Alternative Names
Lymphocyte activation gene 3 protein; CD223
UniProt
P18627
Immunogen
The original antibody was isolated from a human antibody phage display library by bio- panning against human LAG-3.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was able to bind LAG-3 expressed at the surface of HEK-293 cells was evaluated by flow cytometry. The antibody showed binding to activated T cells. The antibody bound to cells expressing rhesus LAG-3 on transiently transfected HEK-293 cells. The affinity of the antibody to human LAG-3 was measured using Bio-Layer Interferometry (Kd = 0.70 nM). The antibody showed inhibitory capacity of the binding of LAG-3 to MHC class II in a dose dependent manner, and was able to completely block binding of LAG-3 to MHC-II at high concentrations (IC50 = 12 nM). The antibody could block the binding of LAG-3 to MHC class II- expressing Daudi cells in a dose-dependent manner by flow cytometry analysis. The antibody was able reverse exhaustion and restore the activity of T cells to secrete IL-2 and IFN-γ upon restimulation at high doses (US20190040136A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
