Cat. No.
MABL-2227
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mycobacterium bovis, Mycobacterium tuberculosis
Clone No.
BJRbL03
From
Recombinant Antibody
Specificity
This antibody is specific for the LAM protein of Mycobacterium tuberculosis but shows cross- reactivity with other Mycobacterium species like M. bovis. M. tuberculosis causes the disease tuberculosis.
Alternative Names
Lipoarabinomannan; Mycobacterium cell wall polysaccharide
UniProt
Immunogen
This antibody was generated by immunizing a rabbit with Mycobacterium tuberculosis H37Rv cell wall.
Application Notes
The ability of the rabbit version of this antibody to bind LAM was determined using ELISA on LAM. Furthermore, the affinity of this antibody to LAM was determined using a ForteBio Octe machine. Finally, the ability of this antibody to distinguish isolates of M. tuberculosis (MTB), M. avium- intracellulare (MAI), M. abscessus, and M. fortuitum-abscessus (MA-MF) was assessed using flow cytometry; it bound all bacteria (Yan et al., 2021; PMID: 32146163). This antibody was used as a capture antibody in a sandwich ELISA (Yan et al., 2023; PMID: 37764103).
Antibody First Published
Yan et al. Generation of mycobacterial lipoarabinomannan-specific monoclonal antibodies and their ability to identify mycobacterium isolates. J Microbiol Immunol Infect. 2021 Jun;54(3):437-446. PMID:32146163
Note on publication
Novel rabbit monoclonal antibodies (mAbs) against LAM for immunodetection purposes were developed.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
