Cat. No.
MABL-2234
Application
IP, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Human, Mouse
Clone No.
A5
From
Recombinant Antibody
Specificity
This antibody recognizes and precipitates laminin, a macromolecule prevalent in the basement membrane responsible for the attachment of cells to each other and forming tissue in the embryonic development of an organism.
Alternative Names
S-LAM gamma; Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin- 6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; S-laminin subunit gamma
UniProt
P11047
Immunogen
The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.
Application Notes
This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID： 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID： 3537540).
Antibody First Published
Ljubimov AV, Afanasjeva AV, Litvinova LV, Senin VM Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies Exp Cell Res. 1986 Aug;165(2)：530-40 PMID:3522258
Note on publication
Describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
