Cat. No.
MABL-2233
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Dog, Rat, Human, Mouse
Clone No.
133A2
From
Recombinant Antibody
Specificity
The antibody recognizes an epitope located between residues 598-611 of lamin A.
Alternative Names
Prelamin-A/C; Lamin-A/C
UniProt
P02545
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with partially purified recombinant human lamin A.
Application Notes
The antibody detected lamin A by western blot analysis (Hozák et al., 1995; PMID:7769007 and Broers et al., 1997; PMID: 9243284). The original format of the antibody strongly stained all tubular epithelium cells of kidney by immunohistochemistry (Broers et al., 1997; PMID: 9243284). The original antibody was used for staining of Sertoli cells, Leydig cells and Peritubular cells in normal testis and parenchyma adjacent to TGCT by immunohistochemistry (Machiels et al., 1997; PMID: 9274531). This antibody was used for detection of lamin A expressed on CHO-K1 cells by flow cytometry (Broers et al., 1999; PMID: 10504295). The original antibody was used to stain lamin A on late apoptotic cell (Broers et al., 2002; PMID: 12553668). The antibody detected laimin A in fibroblast cells by immunofluorescence analysis (van Tienen et al., 2019; PMID: 30420677).
Antibody First Published
Hozák et al. Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells J Cell Sci. 1995 Feb;108 ( Pt 2):635-44. PMID:7769007
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
