Cat. No.
MABL-2232
Application
IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
N10/2
From
Recombinant Antibody
Specificity
This antibody is specific for lambda light chains and does not show cross-reactivity with kappa light chains. It stains B cell follicles in human lymphoid tissues. Lambda light chain is one of the two types of light chains of mammalian immunoglobulins (one Ig has only one type of chains). Usually, the ratio of kappa to lambda is 70:30 but it might be disturbed in various B-cell conditions, such as myeloma.
Alternative Names
IGL; immunoglobulin lambda light chain; lambda chain; lambda; λ immunoglobulin light chain; anti-λ
UniProt
P0DOY2
Immunogen
This antibody was raised by immunising BALB/c mice with purified human IgG.
Application Notes
N10/2 antibody may be utilised in the identification of certain pathologies (mainly associated with B-cells), such as leukemias, plasmacytomas, and some non-Hodgkin's lymphomas. For example, one group used this antibody during an immunohistochemical characterisation of human fetal bone sections engrafted with Waldenström macroglobulinemia cells (Tassone et al., 2005). Another group utilised N10/2 antibody to analyse the light chains expression in post-transplant lymphoproliferative disorders via immunohistochemistry on formalin-fixed, paraffin-embedded tissue samples (Capello et al., 2006).
Antibody First Published
Mason et al. Double immunofluorescence labelling of routinely processed paraffin sections. J Pathol. 2000 Aug;191(4):452-61. PMID:10918222
Note on publication
This article describes the earliest found application of this antibody where it was successfully utilised in immunohistochemical labelling.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
