Cat. No.
MABL-2247
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PR 5C5
From
Recombinant Antibody
Specificity
This antibody recognises human Lewis A antigen, and cross-reacts weakly with Galα1-4Gal and rhamnose.
Alternative Names
LE; FUT3; FUT2; ABO; PR.5C5; pr.5c5
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with mucosal scrapings and cell membrane preparations from normal human large intestine.
Application Notes
This antibody recognises human Lewis A antigen, and has been employed in the immunohistochemical diagnosis of colon disorders, including bowel, small intestine, stomach and colorectal cancers. It has been used in indirect immunoperoxidase staining of formalin-fixed, paraffin-embedded samples of normal colorectal epithelial cells and colon carcinomas (Richman & Bodmer, 1987). It has also been used in indirect immunofluorescence examination of frozen sections of colorectal tissue (Richman & Bodmer, 1987). Its specificity has been confirmed in ELISA analysis (Manimala et al, 2007).
Antibody First Published
Richman & Bodmer Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization. Int J Cancer. 1987 Mar 15;39(3):317-28. PMID:2434440
Note on publication
Describes the original generation of this antibody, and its use in immunohistochemistry experiments
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
