Cat. No.
MABL-2248
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HEA164
From
Recombinant Antibody
Specificity
This antibody is specific for Lewis a,b carbohydrate antigens.
Alternative Names
Blood Group Lewis a/b; lewis a; lewis b
UniProt
Immunogen
This antibody was raised by immunising mice with human carcinoma cells.
Application Notes
This antibody has applications in the detection of normal and neoplastic epithelial cells. This antibody has been shown to react with a glycolipid fraction isolated from colon carcinoma tissue, with no effect of pronase E addition on its reactivity (Moldenhauer et al, 1987). This antibody, when immobilised on the wall of a shear chamber, has been shown to support slow leukocyte rolling (Chen et al, 1997).
Antibody First Published
Moldenhauer et al. Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br J Cancer. 1987 Dec; 56(6): 714–721. PMID:2449234
Note on publication
Describes the original use of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
