Cat. No.
MABL-2249
Application
ICC, IHC
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
291-2G3-A
From
Recombinant Antibody
Specificity
The antibody binds to Lewis X trisaccharide, a carbohydrade pivotal in mediating specific cell- cell interactions, with an affinity of 11 μM.
Alternative Names
Sialyl Lewis X; SLeX; Stage-specific embryonic antigen 1; SSEA-1; CD15
UniProt
Immunogen
This antibody was obtained from mice infected with schistosomes.
Application Notes
The antibody binds specifically to Lewis X, a tetrasaccharide carbohydrate blood-group antigen involved in cell-to-cell adhesion. The antigen is involved in human fertilisation, as it is present on the terminus of N- and O-glycans of the zona pellucida of egg cells, which are recognised by sperm cells This interaction allows fusion and fertilisation to occur. LeX also plays a role in leukocyte tethering as they travel through the bloodstream as it can interact with L-selectin, P-selectin and E-selectin. LeX is also present on neutrophils, mediating phagocytosis and chemotaxis, and is expressed in patients with Hodgkin disease, B- cell chronic lymphocytic leukemia, acute lymphoblastic leukemias and acute nonlymphocytic leukemia. It is also expressed as SSEA-1 on mouse embryonic stem cells. LeX also plays a role in human resistance to the parasitic disease schistosomiasis.
Antibody First Published
van Roon AM, Pannu NS, Hokke CH, Deelder AM, Abrahams JP. Crystallization and preliminary X-ray analysis of an anti-Lewis X Fab fragment with and without its Lewis X antigen. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1306-9. Epub 2003 Jun 27. PMID:12832795
Note on publication
Describes the production of the Fab fragment of the monoclonal antibody 291-2G3- A, used to crystallise the structure of Lewis X.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
