Cat. No.
MABL-2254
Application
FACS, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1-12
From
Recombinant Antibody
Specificity
The antibody is specific for human LgR5. The antibody binds an epitope outside the region of amino acids 22 to 322 of human LgR5.
Alternative Names
LGR5.1-12; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
UniProt
O75473
Immunogen
The original antibody was originated by immunizing rabbits with an LgR5 extracellular domain (ECD) huFc.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format of the antibody could recognise human LgR5 expressed in 293 cells by FACS. The original format of the antibody was used to detect expression of LgR5 on normal human tissues by immunohistochemistry. The antibody stained normal intestinal crypts in the expected pattern. The antibody also showed moderate staining in hair follicle, and weak staining in fallopian tube, endometrium, adrenal gland, and spinal cord. Further, the antibody detected LgR5 in different colon cancer cell lines (SW403, SW948, CACO-2, T84, KM-12, C2BBel, SW1463, SK-CO-1, LOVO) by immunohistochemistry (US20190248889).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
