Cat. No.
MABL-2256
Application
IHC-P, RIA, therapeutic, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SMI41
From
Recombinant Antibody
Specificity
SMI41 reacts with LHRH with an affinity of 10 pM and is specific for its C-terminal pentapeptide (G-L-R-P-G).
Alternative Names
GnRH; LHRH; LNRH; Anti-Gonadotropin-Releasing Hormone; GNRH1; Gonadoliberin; Gonadorelin; luteinizing hormone release hormone; SMI-41; SMI 41
UniProt
P30968
Immunogen
LHRH diacetate tetrahydrate was conjugated to potato acid phosphatase and an emulsion composed of the conjugate and Freunds complete adjuvant was injected into the hind limb of female CAF1 mice. Immune spleen cells were fused with P3-X63-Ag8.653/3 myeloma cells by a modification of the original cell fusion method using polyethylene glycol. A hybridoma screening assays were used to select for high affinity monoclonal antibodies.
Application Notes
SMI-41 has been used in immunocytochemistry experiments to detect the expression of mature LHRH in the brain detect cells containing mature LHRH peptide (Avigdor 2004). SMI-41 also has been expressed in mice via injection of a replication-incompetent, recombinant adeno-associated virus designed to express the antibody. This has been used to cofer long-term infertility in male and female mice (Li 2015).
Antibody First Published
Knapp and Sternberger High Affinity Monoclonal Antibodies to Luteinizing Hormone-releasing Hormone J Neuroimmunol. 1984 Aug;6(5):361-71. PMID:6378971
Note on publication
Describes the generation of monoclonal antibodies against Gonadotropin-releasing hormone.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
