Cat. No.
MABL-2257
Application
crystallization, EIA, PIH, ELISA
Isotype
Engineer antibody
Species Reactivity
Gram-negative bacteria
Clone No.
S1-15
From
Recombinant Antibody
Specificity
The antibody recognizes the carbohydrate backbone of lipid A. The antibody binds to the 4'- monophosphoryl GlcN disaccharide. The antibody does not cross react with lipopolysaccharides (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid component of LPS contained in gram-negative bacteria. Lipid A is the endotoxic principle of LPS.
Alternative Names
lipA
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with lipA.
Application Notes
In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993).
Antibody First Published
Kuhn et al. Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A. Infect Immun. 1992 Jun;60(6):2201-10. PMID:1375194
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
