Cat. No.
MABL-2258
Application
SPR, TLC, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Chlamydophila psittaci
Clone No.
S69-4
From
Recombinant Antibody
Specificity
This antibody is specific for Kdo4, a Chlamydophila psittaci Lipopolysaccharide (LPS) tetrasaccharide (3-deoxy-alpha-d-manno-oct-2-ulopyranosonic acid (Kdo)) of the sequence Kdo(2-->8)[Kdo(2-->4)] Kdo(2-->4)Kdo. This antibody specifically binds to the LPS of Chlamydophila psittaci but not to LPS from Chlamydophila pneumoniae or Chlamydia trachomatis. Furthermore, it binds exclusively to the LPS and O-deacylated LPS of Chlamydophila psittaci.
Alternative Names
LPS; Kdo(2→8)[Kdo(2→4)]Kdo(2→4) Kdo; Kdo4; branched Kdo4 tetrasaccharide; Chlamydophila psittaci Lipopolysaccharide tetrasaccharide; 3-deoxy-alpha-d-manno-oct-2- ulopyranosonic acid
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with a synthetic neoglycoconjugate antigen — Kdo(2-->8)[Kdo(2-->4)]Kdo(2-->4) Kdo-BSA (Kdo4-BSA).
Application Notes
This antibody demonstrated high affinity and specificity for structures containing the branched Kdo4 tetrasaccharide or the 2→4/2→4Kdo3 trisaccharide, enabling differentiation of Chlamydophila psittaci from other chlamydial species. Furthermore, it shows potential for detecting individual molecular lipopolysaccharide (LPS) species and studying the enzymology of Kdo transferases. Its binding affinity to neoglycoconjugate antigens was assessed using ELISA checkerboard titrations. It displayed comparable affinity for 2→4/2→4Kdo3-BSA and Kdo4-BSA and weak binding was observed with 2→4Kdo2-BSA, 2→8Kdo2-BSA, and 2→8/2→4Kdo3-BSA, but only at high antibody and antigen concentrations. This antibody was also tested against BSA glycoconjugates containing 4'-monophosphoryl derivatives of deacylated C. psittaci LPS. It demonstrated similar reactivity with 2→4/2→4Kdo3-GlcNAc2-4P-BSA and Kdo4- GlcNAc2-4P-BSA. Further experiments confirmed its binding to isolated LPS and whole bacteria. Inhibition assays using free oligosaccharides as inhibitors in ELISA demonstrated that the antibody achieved 50% inhibition with 2→4/2→4Kdo3-allyl, Kdo4-allyl, and Kdo4-GlcNAc2-P2 at concentrations of 4.4 µM, 1.7 µM, and 2.1 µM, respectively.
Antibody First Published
Müller-Loennies et al. A monoclonal antibody against a carbohydrate epitope in lipopolysaccharide differentiates Chlamydophila psittaci from Chlamydophila pecorum, Chlamydophila pneumoniae, and Chlamydia trachomatis Glycobiology. 2006 Mar;16(3):184-96. doi: 10.1093/glycob/cwj055 PMID:16282606
Note on publication
The original publication discusses the development of a monoclonal antibody that can differentiate Chlamydophila psittaci from other Chlamydophila species and Chlamydia trachomatis based on a specific carbohydrate epitope in the lipopolysaccharide.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
