Cat. No.
MABL-2269
Application
therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Gram Positive Bacteria
Clone No.
BSYX-A110 (Pagibaximab)
From
Recombinant Antibody
Specificity
This antibody targets the lipoteichoic acid (LTA) which is a major cell wall component of gram positive bacteria. LTA plays an important role in the initiation and progression of bacterial infection, inflammation, and septic shock. This antibody is also reported to bind whole bacteria for e.g. S. epidermidis (types I, II & III), S. hemolyticus, S. hominus, and two serotypes of S. aureus but not to gram negative bacteria like E. coli.
Alternative Names
LTA; Staphylococcus epidermidis lipoteichoic acid
UniProt
Immunogen
The original antibody was generated by immunizing mice with whole bacteria strain Hay Streptococcus epidermidis.
Application Notes
This antibody is also known as PMAB, A110 and Hu96-110. This humanized chimeric antibody is derived from murine antibody M110 and is reported to bind whole bacteria and enhance phagocytosis and killing of the bacteria in vitro and enhance protection from lethal infection in vivo. It was originally developed for the prevention of staphylococcal sepsis in infants with low body weight. The serum half-life of the antibody for VLBW infants was 20.5 ± 6.8 days. Pagibaximab enhanced serum opsonophagocytic activity in infants with no signs of immunogenecity of pagibaximab. All staphylococci causing sepsis were opsonizable by pagibaximab (PMID:19380597). Another study reported that three once- a-week 90 or 60 mg/kg pagibaximab infusions, in high-risk neonates, seemed safe and well tolerated (PMID: 21788224). The binding activity of the antibody to bacteria was determined using whole cell ELISA. The opsonic activity of the antibody was tested with a neutrophil mediated bacteriocidal assay.
Antibody First Published
Weisman et al. Antibody for the prevention of neonatal noscocomial staphylococcal infection: a review of the literature. Arch Pediatr. (2007); 14 Suppl 1:S31-4. PMID:17939955
Note on publication
Describes the potential use of this antibody in the prevention and treatment of neonatal noscocomial staphylococcal infections.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
