Cat. No.
MABL-2271
Application
ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Epstein-Barr virus
Clone No.
HLEAFab
From
Recombinant Antibody
Specificity
This antibody binds to the LMP1 extracellular domain in native confirmation.
Alternative Names
LMP-1; p63; Latent membrane protein 1; protein p63
UniProt
P03230
Immunogen
The original antibody was generated from a human Fab phage library after panning against HNE2 cell line and HNE2/LMP1 cell line, as well as on immobilized LMP1 extracellular domains. The phages with specific binding for extracellular domains were selected after seven rounds of panning.
Application Notes
This antibody binds to the extracellular domain of LMP1, which was confirmed with ELISA. The ability of the expressed HLEAFab to specifically bind to native LMP1 on the HNE2/LMP1 cell surface was further confirmed by Cell ELISA. The binding was also confirmed using flow cytometry and immunofluorescence staining. An immunoconjugate fof HLEAFab and mitomycin C (HLEAFab-MMC) inhibits proliferation and induced apoptosis in nasopharyngeal carcinoma (NPC) cell line HNE2/LMP1 in vitro and is also found to show inhibition of growth of NPC xenografts in nude mice (PMID: 22169768). HLEAFab works synergistically with mitomycin C to inhibit growth of nasopharyngeal carcinoma growth in vivo through inducing apoptosis and downregulating vascular endothelial growth factor (VEGF). Immunohistochemical staining showed that VEGF expression in NPC xenografts was significantly inhibited in the combination group compared to the Fab (PMID: 22408448). HLEAFab is recommended to be used to detect LMP1 expression in NPC tissues using immunohistochemical staining. Positive staining of LMP1 was observed in the membrane and cytoplasm of NPC cells (PMID: 23760364).
Antibody First Published
Chen et al. A Human Fab-Based Immunoconjugate Specific for the LMP1 Extracellular Domain Inhibits Nasopharyngeal Carcinoma Growth In Vitro and In Vivo. Mol Cancer Ther. (2012); 11(3); 594-603. PMID:22169768
Note on publication
Describes the generation of the Fab and an immunoconjugate of Fab with mitomycin C (MMC) and studies the effects of HLEAFab-MMC on proliferation and apoptosis in NPC cell lines HNE2/LMP1 and the inhibition rate of growth of NPC xenografts in nude mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
