Cat. No.
MABL-2277
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Burkholderia pseudomallei, Burkholderia thailandensis
Clone No.
CK3 (BP1 7F7)
From
Recombinant Antibody
Specificity
This antibody binds the surface lipopolysaccharide of Burkholderia pseudomallei and Burkholderia thailandensis. Burkholderia pseudomallei (BP), is Recently, BP has been recognized as a major cause of community-acquired septicemia, resulting in significant mortalitythe causative agent of melioidosis (Whitmore’s disease), is a gram-negative, facultative anaerobic, motile bacillus commonly found in the soil and stagnant waters. BP infection is often due to either direct inoculation into wounds and skin abrasions or inhalation of contaminated materials. Recently, BP has been recognized as a major cause of community- acquired septicemia, resulting in significant mortality.
Alternative Names
Lipopolysaccharide; Burkholderia spp; Burkholderia pseudomallei; BP; Burkholderia thailandensis, melioidosis; Whitmore’s disease
UniProt
Immunogen
The original antibody was generated by immunizing mice with various antigen preparations from B. pseudomallei (ATCC 23343).
Application Notes
This antibody can be used for the detection of heat killed, sodium periodate treated, proteinase K treated or 0.02% SDS treated Burkholderia pseudomallei and Burkholderia thailandensis in a sample using ELISA. It was reported that this antibody could recognize 34, 38∼52 kDa polypeptide antigens from Burkholderia pseudomallei and 34, 38∼60 kDa polypeptide antigens from Burkholderia thailandensis in a western blot assay (PMID: 21573027).
Antibody First Published
Yong-Kim et al. Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system. PLoS One. 2011 May 9;6(5):e19867. PMID:21573027
Note on publication
Describes the generation and characterization of chimeric antibodies against Burkholderia pseudomallei and B. mallei.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
