Cat. No.
MABL-2285
Application
functional assay, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
HK1.4
From
Recombinant Antibody
Specificity
This antibody is specific for Ly6 and shows no cross reactivity. Ly6 shows acetylcholine receptor binding activity.
Alternative Names
Lymphocyte antigen 6C1; Ly6c1
UniProt
P0CW02
Immunogen
The antibody was raised by immunizing a Lewis rat by a series of four monthly i.p. injections of approximately 30 X 10" L3 cloned CTL cells.
Application Notes
A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild- type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). Neonatal murine calvaria-derived osteoblasts were stained for flow cytometry using this antibody (Horowitz et al, 1994; pmid:7520861).
Antibody First Published
Havran et al. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes J Immunol. 1988 Feb 15;140(4):1034-42. PMID:3257767
Note on publication
The paper describes the generation of the antibody is rats by immunizing them with L3 cloned CTL cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
