Cat. No.
MABL-2289
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
6B9
From
Recombinant Antibody
Specificity
This antibody is highly specific for lycopene and does not recognize other carotenoids such as lutein. Lycopene is a naturally occurring red pigment, which belongs to the family of carotenoids. It is found in tomatoes, watermelon and papaya. Lycopene is one of the most powerful antioxidants, belonging to the tetraterpene group, whose anti-radical activity exceeds the antioxidant potential of tocopherol, beta- carotene and ascorbic acid. The highly unsaturated chemical structure of lycopene predetermines its ability to react with many substances containing or generating singlet molecular oxygen such as peroxyl radicals, hydrogen peroxide and hypochlorite.
Alternative Names
Carotene; carotenoid; C40H56; CAS: 502-65-8; CHEBI:15948
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with lycopene-gold conjugated colloidal particles.
Application Notes
The binding reactivity of the original antibody towards lycopene was determined using ELISA. This antibody was also able to recognize lycopene inclusions in the settings of indirect immunofluorescence experiments. This antibody can also be used for the visualization of lycopene distribution within cultured human hepatocytes and alveolar macrophages which were pre-incubated with this carotenoid for 48 hours (WO2018115885).
Antibody First Published
Petyaev et al. Antibodies against carotenoids (WO2018115885).
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
