Cat. No.
MABL-2293
Application
crystallization, SPR
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
cAbHul6
From
Recombinant Antibody
Specificity
The antibody is specificity for human lysozyme. It does not cross react with hen lysozyme.
Alternative Names
1,4-beta-N-acetylmuramidase C; cAb-Hul6; NbHuL6
UniProt
P61626
Immunogen
Phage libraries were generated using the mRNA extracted from the lymphocytes of immunized dromedaries. The VHH antibody was isolated by biopanning from the libraries.
Application Notes
The binding affinity of the VHH fragment for human lysozyme was measured by surface plasmon resonance (Kd = 0.7 nM) (Dumoulin et al., 2002; PMID: 11847273). The antibody stabilized an unstable human lysozyme mutant that forms fibrils and amyloids.The crystal structure of the original version of the antibody in complex with human lysozyme was determined (Dumoulin et al., 2003; PMID: 12917687). The original antibody was humanized and the binding affinity for human lysozyme was measured by surface plasmon resonance (Kd = 2.36 nM). The crystal structure of the humanized version of the antibody in complex with human lysozyme was determined (Vincke et al., 2009; PMID:19010777).
Antibody First Published
Dumoulin et al. Single-domain antibody fragments with high conformational stability. Protein Sci. 2002 Mar;11(3)；500-15. do:10.1110/ps.34602. PMID:11847273
Note on publication
The original paper describes the generation and characterization of the antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
